Diffusion‐based size determination of solute particles: a method adapted for postsynaptic proteins
  • Metaadatok
Tartalom: https://publikacio.ppke.hu/id/eprint/2754/
Archívum: PPKE Publikáció Repozitórium
Gyűjtemény: Állapot = Benyújtva
Szakterület = 01. Természettudományok: 01.06. Biológiai tudományok: 01.06.03. Biokémia és molekuláris biológia
Típus = Folyóiratcikk
Cím:
Diffusion‐based size determination of solute particles: a method adapted for postsynaptic proteins
Létrehozó:
Szabó András László
Nagy-Kanta Eszter
Varga Soma
Kassainé Jáger Edit
Pongor Csaba István
Laki Mária
Laki András József
Gáspári Zoltán
Dátum:
2025
Téma:
01.06.03. Biokémia és molekuláris biológia
Tartalmi leírás:
The postsynaptic density (PSD) is a complex, multilayered protein network largely situated on the internal surface of the postsynaptic membrane. It is the first processing unit for incoming synaptic signals, and changes in its internal structure are associated with synaptic strength and plasticity. These structural changes are largely governed by multivalent interactions between its components. The in vitro characterization of such complexes requires unbiased methods that can be used to estimate the size of the emerging assemblies for systems with multiple possible stoichiometries. Here, we present an experimental method for detecting specific PSD proteins as well as their complexes based on their diffusion in a microfluidic environment. The method requires a fluorescent labeling technique that does not disrupt the function of labeled proteins, a microfluidic device that can maintain laminar flow for protein solutions, a microscope that can record the fluorescent signal emitted by these solutions, and an analytic software package that can process the collected experimental data and convert them into approximate particle sizes. We demonstrate the applicability of our method on protein constructs of various postsynaptic proteins, including the multivalent assembly between GKAP and LC8.
Nyelv:
angol
angol
Típus:
Folyóiratcikk
PeerReviewed
Formátum:
text
Azonosító:
Szabó András László; Nagy-Kanta Eszter; Varga Soma; Kassainé Jáger Edit; Pongor Csaba István; Laki Mária; Laki András József; Gáspári Zoltán: Diffusion‐based size determination of solute particles: a method adapted for postsynaptic proteins. FEBS OPEN BIO. pp. 1-16. ISSN 2211-5463 (2025) (Benyújtva)
MTMT:36314112 10.1002/2211-5463.70111
Kapcsolat:
MTMT:36314112 10.1002/2211-5463.70111