A ‘torn bag mechanism’ of small extracellular vesicle release via limiting membrane rupture of en bloc released amphisomes (amphiectosomes)
  • Metaadatok
Tartalom: https://real.mtak.hu/216633/
Archívum: REAL
Gyűjtemény: Status = Published
Subject = Q Science / természettudomány: QH Natural history / természetrajz: QH301 Biology / biológia
Type = Article
Cím:
A ‘torn bag mechanism’ of small extracellular vesicle release via limiting membrane rupture of en bloc released amphisomes (amphiectosomes)
Létrehozó:
Visnovitz, Tamás
Lenzinger, Dorina
Koncz, Anna
Vizi, Péter M
Bárkai, Tünde
Visnovitzné Dr Vukman, Krisztina
Galinsoga, Alicia
Németh, Krisztina
Fletcher, Kelsey Aine
Komlósi, Zsolt
Cserép, Csaba
Dénes, Ádám
Lőrincz, Péter
Valcz, Gábor
Buzás, Edit Irén
Dátum:
2025
Téma:
QH301 Biology / biológia
Tartalmi leírás:
Recent studies showed an unexpected complexity of extracellular vesicle (EV) biogenesis pathways. We previously found evidence that human colorectal cancer cells in vivo release large multivesicular body-like structures en bloc. Here, we tested whether this large EV type is unique to colorectal cancer cells. We found that all cell types we studied (including different cell lines and cells in their original tissue environment) released multivesicular large EVs (MV-lEVs). We also demonstrated that upon spontaneous rupture of the limiting membrane of the MV-lEVs, their intraluminal vesicles (ILVs) escaped to the extracellular environment by a ‘torn bag mechanism’. We proved that the MV-lEVs were released by ectocytosis of amphisomes (hence, we termed them amphiectosomes). Both ILVs of amphiectosomes and small EVs separated from conditioned media were either exclusively CD63 or LC3B positive. According to our model, upon fusion of multivesicular bodies with autophagosomes, fragments of the autophagosomal inner membrane curl up to form LC3B positive ILVs of amphisomes, while CD63 positive small EVs are of multivesicular body origin. Our data suggest a novel common release mechanism for small EVs, distinct from the exocytosis of multivesicular bodies or amphisomes, as well as the small ectosome release pathway.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Visnovitz, Tamás and Lenzinger, Dorina and Koncz, Anna and Vizi, Péter M and Bárkai, Tünde and Visnovitzné Dr Vukman, Krisztina and Galinsoga, Alicia and Németh, Krisztina and Fletcher, Kelsey Aine and Komlósi, Zsolt and Cserép, Csaba and Dénes, Ádám and Lőrincz, Péter and Valcz, Gábor and Buzás, Edit Irén (2025) A ‘torn bag mechanism’ of small extracellular vesicle release via limiting membrane rupture of en bloc released amphisomes (amphiectosomes). ELIFE, 13. ISSN 2050-084X
Kapcsolat:
MTMT:35846439 10.7554/eLife.95828
Létrehozó:
cc_by