16S rRNA sequencing reveals microbiota differences in orthopedic implants between aseptic loosening and prosthetic joint infection cases
  • Metaadatok
Tartalom: https://real.mtak.hu/213934/
Archívum: REAL
Gyűjtemény: Status = Published
Subject = R Medicine / orvostudomány: R1 Medicine (General) / orvostudomány általában
Subject = Q Science / természettudomány: QR Microbiology / mikrobiológia
Type = Article
Cím:
16S rRNA sequencing reveals microbiota differences in orthopedic implants between aseptic loosening and prosthetic joint infection cases
Létrehozó:
Cetiner, Omer Faruk
Hora, Mehmet
Kafadar, Ibrahim Halil
Ulu Kilic, Aysegul
Gundogdu, Aycan
Kiadó:
Akadémiai Kiadó
Dátum:
2024
Téma:
QR Microbiology / mikrobiológia
R1 Medicine (General) / orvostudomány általában
Tartalmi leírás:
Prosthetic joint infection (PJI) and aseptic loosening (AL) are common complications of total joint arthroplasty. An accumulation of evidence indicates the presence of microbial communities on prosthetic implants, but the overall microbial profile is unclear. In this study, we aimed to investigate the differences in the microbial composition of prosthetic implants obtained from PJI and AL patients using the 16S rRNA sequencing method. Patients who underwent revision hip, knee, or shoulder arthroplasty caused by PJI (n = 20) or AL (n = 10) were enrolled in the study. 16S rRNA sequencing targeting the V3–V4 region was performed on the microbial specimens collected from synovial fluid, periprosthetic deep-tissue, and biofilm during the revision surgery. The sequenced raw data were analysed for microbial composition and ecological and differential abundance analyses using bioinformatics tools. The AL group had relatively balanced and higher diversity, with Staphylococcus, Streptococcus, and Veillonella being prominent. In the PJI group, Staphylococcus and Pseudomonas were predominant, especially in deep-tissue samples and biofilm samples, respectively. The differential abundance analysis identified 15 and 2 distinctive taxa in the AL and PJI groups, respectively. Our findings provided preliminary insights supporting the existence of periprosthetic microbiota in orthopedic implants and explaining the differences in microbial composition between the AL and PJI groups.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Cetiner, Omer Faruk and Hora, Mehmet and Kafadar, Ibrahim Halil and Ulu Kilic, Aysegul and Gundogdu, Aycan (2024) 16S rRNA sequencing reveals microbiota differences in orthopedic implants between aseptic loosening and prosthetic joint infection cases. Acta Microbiologica et Immunologica Hungarica, 71 (2). pp. 172-181. ISSN 1217-8950
Kapcsolat:
MTMT:35704525 10.1556/030.2024.02265