Integrative analysis of yeast colony growth

Gaizer Tünde Éva

Juhász János

Pillér Bíborka

Szakadáti Helga

Pongor Csaba István

Csikász-Nagy Attila

2024

01.06.03.03. Biokémia

01.06.03.04. Molekuláris biológia

Yeast colonies are routinely grown on agar plates in everyday experimental settings to understand basic molecular processes, produce novel drugs, improve health, and so on. Standardized conditions ensure these colonies grow in a reproducible fashion, while in nature microbes are under a constantly changing environment. Here we combine the power of computational simulations and laboratory experiments to investigate the impact of non-standard environmental factors on colony growth. We present the developement and parameterization of a quantitative agent-based model for yeast colony growth to reproduce measurements on colony size and cell number in a colony at non-standard environmental conditions. Specifically, we establish experimental conditions that mimic the effects of humidity changes and nutrient gradients. Our results show how colony growth is affected by moisture changes, nutrient availability, and initial colony inoculation conditions. We show that initial colony spread, not initial cell number have higher impact on the final size and cell number of colonies. Parameters of the model were identified by fitting these experiments and the fitted model gives guidance to establish conditions which enable unlimited growth of yeast colonies.

angol

angol

Folyóiratcikk

PeerReviewed

text

https://publikacio.ppke.hu/id/eprint/1768/1/communicationsbiology2024.pdf

Gaizer Tünde Éva; Juhász János; Pillér Bíborka; Szakadáti Helga; Pongor Csaba István; Csikász-Nagy Attila: Integrative analysis of yeast colony growth. COMMUNICATIONS BIOLOGY, 7 (1). ISSN 2399-3642 (2024)

MTMT:34837874 10.1038/s42003-024-06218-1

https://publikacio.ppke.hu/id/eprint/1768/

https://doi.org/10.1038/s42003-024-06218-1

MTMT:34837874 10.1038/s42003-024-06218-1