A newly identified specific biological activity of glyphosate – inhibition of RGD-binding integrins
  • Metaadatok
Tartalom: https://real.mtak.hu/203945/
Archívum: REAL
Gyűjtemény: Status = Published
Subject = S Agriculture / mezőgazdaság: S1 Agriculture (General) / mezőgazdaság általában
Type = Book Section
Subject = S Agriculture / mezőgazdaság: SB Plant culture / növénytermesztés: SB975 Plant protection / növényvédelem
Cím:
A newly identified specific biological activity of glyphosate – inhibition of RGD-binding integrins
Létrehozó:
Gémes, Borbála Letícia
Takács, Eszter
Székács, Inna
Farkas, Enikő
Horváth, Róbert
Klátyik, Szandra
Székács, András
Kiadó:
Szegedi Tudományegyetem
Dátum:
2022
Téma:
S1 Agriculture (General) / mezőgazdaság általában
SB975 Plant protection / növényvédelem
Tartalmi leírás:
In this study we investigated the inhibitory effect of the widely used broad-spectrum herbicide active ingredient glyphosate and its related analogues on αVβ3 integrin binding to the shortest oligopeptide recognizing motif of integrins, the arginine-glycine-aspartic acid (RGD) sequence. Integrin binding characteristics were assessed in a modified enzyme linked immunosorbent assay (ELISA) and by a label-free optical biosensor technique. At 22 mM, glyphosate reached full inhibition of αVβ3, and the inhibitory activity of its main metabolite, aminomethylphosphonic acid (AMPA) was also above 95%, while another environmentally relevant metabolite, sarcosine exerted only a weaker effect, approximately 35% inhibition. In turn, the half maximal inhibitory concentration (IC50) of glyphosate and AMPA were reported to be 2.7±0.5 mM and 1.3±0.2 mM, respectively. The inhibitory effects of the other related compounds investigated (acetylglycine, glycine and iminodiacetic acid) at the same concentration, 22 mM were below 50%. Inhibitory effects on cell adhesion to RGD-modified surfaces by whole cells containing several types of RGD-binding integrins including αVβ3 were detected using the biosensor technique, where the integrin antagonist activity of glyphosate was also demonstrated.
Nyelv:
angol
Típus:
Book Section
PeerReviewed
info:eu-repo/semantics/bookPart
Formátum:
text
Azonosító:
Gémes, Borbála Letícia and Takács, Eszter and Székács, Inna and Farkas, Enikő and Horváth, Róbert and Klátyik, Szandra and Székács, András (2022) A newly identified specific biological activity of glyphosate – inhibition of RGD-binding integrins. In: Proceedings of the 28th International Symposium on Analytical and Environmental Problems. Szegedi Tudományegyetem, Szeged, pp. 147-151. ISBN 9789633069042
Kapcsolat:
MTMT:33291423