A New Application of Spin and Fluorescence Double-Sensor Molecules
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Tartalom: http://real.mtak.hu/173414/
Archívum: REAL
Gyűjtemény: Status = Published
Subject = Q Science / természettudomány: QD Chemistry / kémia
Subject = Q Science / természettudomány: QD Chemistry / kémia: QD02 Physical chemistry / fizikai kémia
Type = Article
Cím:
A New Application of Spin and Fluorescence Double-Sensor Molecules
Létrehozó:
Bencze, Florián
Bognár, Balázs
Kálai, Tamás
Kollár, László
Nagymihály, Zoltán
Kunsági-Máté, Sándor
Kiadó:
MDPI
Dátum:
2023
Téma:
QD Chemistry / kémia
QD02 Physical chemistry / fizikai kémia
Tartalmi leírás:
EPR imaging techniques are known to be successful tools for mapping living bodies, especially because of the high transparency of tissues in the microwave range. This technique assumes the presence of radicals whose in vivo transport is also controlled by serum albumins. Accordingly, in this study, the interactions between 3-hydroxymethyl-1-oxyl-4-(pyren-1-yl)-2,2,5,5-tetramethyl-2,5- dihydro-1H-pyrrole radical and the human serum albumin molecules were investigated. To clarify the adsorption processes of this radical onto the surface of human serum albumin (HSA), the interaction of the OMe derivative of the radical was also examined parallel with the studies on the radical— HSA interactions. Considering the solubility issues and also to modulate the transport, inclusion complexes of the radical with a cavitand derivative were also studied. The latter interactions were observed through fluorescence spectroscopy, fluorescence polarization, and by EPR spectroscopy. As a double-sensor molecule, we found that the fluorophore nitroxide is a good candidate as it gave further information about host-guest interactions (fluorescence, fluorescence polarization, and EPR). We also found that in the presence of a cavitand, a complex with greater stability was formed between the sensor molecule and the human serum albumin. Based on these observations, we can conclude that applying this double-sensor (spin, fluorescent) molecule is useful in cases when different interactions can affect the EPR measurements.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Bencze, Florián and Bognár, Balázs and Kálai, Tamás and Kollár, László and Nagymihály, Zoltán and Kunsági-Máté, Sándor (2023) A New Application of Spin and Fluorescence Double-Sensor Molecules. MOLECULES, 28 (7). No-2978. ISSN 1420-3049
Kapcsolat:
Létrehozó:
cc_by
info:eu-repo/semantics/openAccess