A Bioorthogonal Double Fluorogenic Probe to Visualize Protein–DNA Interaction
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Tartalom: http://real.mtak.hu/149418/
Archívum: REAL
Gyűjtemény: Status = Published
Subject = Q Science / természettudomány: QD Chemistry / kémia
Subject = Q Science / természettudomány: QD Chemistry / kémia: QD04 Organic chemistry / szerves kémia
Type = Article
Cím:
A Bioorthogonal Double Fluorogenic Probe to Visualize Protein–DNA Interaction
Létrehozó:
Kormos, Attila
Egyed, Alexandra
Olvany, Jasmine M.
Szatmári, Ágnes
Biró, Adrienn
Csorba, Zsóka
Kele, Péter
Németh, Krisztina
Kiadó:
MDPI
Dátum:
2022-01-17
Téma:
QD Chemistry / kémia
QD04 Organic chemistry / szerves kémia
Tartalmi leírás:
Two sets of bioorthogonally applicable, double fluorogenic probes, capable of sensing DNA–protein interactions, were prepared by installing an azide or tetrazine motif onto structurally fluorogenic, DNA sensitive frames. Installation of these bioorthogonal functions onto DNA intercalating dyes furnished these scaffolds with reactivity based fluorogenicity, rendering these probes double-fluorogenic, AND-type logic switches that require the simultaneous occurrence of a bioorthogonal reaction and interaction with DNA to trigger high intensity fluorescence. The probes were evaluated for double fluorogenic behavior in the presence/absence of DNA and a complementary bioorthogonal function. Our studies revealed that azide and tetrazine appending thiazole orange frames show remarkable double fluorogenic features. One of these probes, a membrane permeable tetrazine modified thiazole orange derivative was further tested in live cell labeling studies. Cells expressing bioorthogonalized DNA-binding proteins showed intensive fluorescence characteristics of the localization of the proteins upon treatment with our double fluorogenic probe. On the contrary, labeling similarly bioorthogonalized cytosolic proteins did not result in the appearance of the fluorescence signal. These studies suggest that such double-fluorogenic probes are indeed capable of sensing DNA–protein interactions in cells.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Kormos, Attila and Egyed, Alexandra and Olvany, Jasmine M. and Szatmári, Ágnes and Biró, Adrienn and Csorba, Zsóka and Kele, Péter and Németh, Krisztina (2022) A Bioorthogonal Double Fluorogenic Probe to Visualize Protein–DNA Interaction. CHEMOSENSORS, 10 (1). p. 37. ISSN 2227-9040
Kapcsolat:
10.3390/chemosensors10010037
Létrehozó:
cc_by