A case study of salivary microbiome in smokers and non-smokers in Hungary: analysis by shotgun metagenome sequencing
  • Metaadatok
Tartalom: http://real.mtak.hu/120244/
Archívum: REAL
Gyűjtemény: Status = Published
Subject = Q Science / természettudomány: QR Microbiology / mikrobiológia
Type = Article
Cím:
A case study of salivary microbiome in smokers and non-smokers in Hungary: analysis by shotgun metagenome sequencing
Létrehozó:
Wirth, Roland
Maróti, Gergely
Mihók, Róbert
Simon-Fiala, Donát
Antal, Márk Ádám
Pap, Bernadett
Nagy-Demcsák, Anett
Minárovits, János
Kovács, Kornél Lajos
Kiadó:
Taylor & Francis
Dátum:
2020
Téma:
QR Microbiology / mikrobiológia
Tartalmi leírás:
Objective To investigate the role of cigarette smoking in disease-development through altering the composition of the oral microbial community. Periodontitis and oral cancer are highly prevalent in Hungary; therefore, the salivary microbiome of smoker and non-smoker Hungarian adults was characterized. Methods Shotgun metagenome sequencing of salivary DNA samples from 22 individuals (11 non-smokers and 11 current smokers) was performed using the Ion Torrent PGMTM platform. Quality-filtered reads were analysed by both alignment-based sequence similarity searches and genome-centric binning. Results Prevotella, Veillonella and Streptococcus were the predominant genera in the saliva of both groups. Although the overall composition and diversity of the microbiota were similar, Prevotella was significantly more abundant in salivary samples of current smokers compared to non-smokers. Members of the genus Prevotella were implicated in the development of inflammatory diseases and oral cancer. The abundance of the genus Megasphaera also increased in current smokers, whereas the genera Neisseria, Oribacterium, Capnocytophaga and Porphyromonas were significantly reduced. The data generated by read-based taxonomic classification and genome-centric binning mutually validated the two distinct metagenomic approaches. Conclusion Smoking-associated dysbiosis of the salivary microbiome in current cigarette smokers, especially increased abundance of Prevotella and Megasphaera genera, may facilitate disease development.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Wirth, Roland and Maróti, Gergely and Mihók, Róbert and Simon-Fiala, Donát and Antal, Márk Ádám and Pap, Bernadett and Nagy-Demcsák, Anett and Minárovits, János and Kovács, Kornél Lajos (2020) A case study of salivary microbiome in smokers and non-smokers in Hungary: analysis by shotgun metagenome sequencing. JOURNAL OF ORAL MICROBIOLOGY, 12 (1). ISSN 2000-2297
Kapcsolat:
MTMT:31336753 doi: 10.1080/20002297.2020.1773067