8-Oxoguanine DNA glycosylase-1-mediated DNA repair is associated with Rho GTPase activation and α-smooth muscle actin polymerization
  • Metaadatok
Tartalom: http://dx.doi.org/10.1016/j.freeradbiomed.2014.03.030
Archívum: REAL
Gyűjtemény: Status = Published
Subject = Q Science / természettudomány: Q1 Science (General) / természettudomány általában
Type = Article
Cím:
8-Oxoguanine DNA glycosylase-1-mediated DNA repair is associated with Rho GTPase activation and α-smooth muscle actin polymerization
Létrehozó:
Luo, Jixian
Hosoki, Koa
Bácsi, Attila
Radak, Zsolt
Hegde, Muralidhar L.
Sur, Sanjiv
Hazra, Tapas K.
Brasier, Allan R.
Ba, Xueqing
Boldogh, István
Dátum:
2014
Téma:
Q1 Science (General) / természettudomány általában
Tartalmi leírás:
Reactive oxygen species (ROS) are activators of cell signaling and modify cellular molecules, including DNA. 8-Oxo-7,8-dihydroguanine (8-oxoG) is one of the prominent lesions in oxidatively damaged DNA, whose accumulation is causally linked to various diseases and aging processes, whereas its etiological relevance is unclear. 8-OxoG is repaired by the 8-oxoguanine DNA glycosylase-1 (OGG1)-initiated DNA base excision repair (BER) pathway. OGG1 binds free 8-oxoG and this complex functions as an activator of Ras family GTPases. Here we examined whether OGG1-initiated BER is associated with the activation of Rho GTPase and mediates changes in the cytoskeleton. To test this possibility, we induced OGG1- initiated BER in cultured cells and mouse lungs and used molecular approaches such as active Rho pull- down assays, siRNA ablation of gene expression, immune blotting, and microscopic imaging. We found that OGG1 physically interacts with Rho GTPase and, in the presence of 8-oxoG base, increases Rho–GTP levels in cultured cells and lungs, which mediates α-smooth muscle actin (α-SMA) polymerization into stress fibers and increases the level of α-SMA in insoluble cellular/tissue fractions. These changes were absent in cells lacking OGG1. These unexpected data and those showing that 8-oxoG repair is a lifetime process suggest that, via Rho GTPase, OGG1 could be involved in the cytoskeletal changes and organ remodeling observed in various chronic diseases.
Típus:
Article
PeerReviewed
Formátum:
text
Azonosító:
Luo, Jixian and Hosoki, Koa and Bácsi, Attila and Radak, Zsolt and Hegde, Muralidhar L. and Sur, Sanjiv and Hazra, Tapas K. and Brasier, Allan R. and Ba, Xueqing and Boldogh, István (2014) 8-Oxoguanine DNA glycosylase-1-mediated DNA repair is associated with Rho GTPase activation and α-smooth muscle actin polymerization. Free Radical Biology and Medicine, 73. pp. 430-438. ISSN 08915849
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