“Fishing and hunting”– Selective immobilization of a recombinant phenylalanine ammonia-lyase from fermentation media
  • Metaadatok
Tartalom: http://real.mtak.hu/103991/
Archívum: REAL
Gyűjtemény: Status = Published
Subject = Q Science / természettudomány: QH Natural history / természetrajz: QH301 Biology / biológia
Subject = Q Science / természettudomány: QD Chemistry / kémia: QD04 Organic chemistry / szerves kémia
Type = Article
Cím:
“Fishing and hunting”– Selective immobilization of a recombinant phenylalanine ammonia-lyase from fermentation media
Létrehozó:
Sánta-Bell, Evelin
Molnár, Zsófia Klára
Varga, Andrea
Nagy, Flóra
Hornyánszky, Gábor
Paizs, Csaba
Balogh Weiser, Diána
Poppe, László
Kiadó:
MDPI
Dátum:
2019
Téma:
QD04 Organic chemistry / szerves kémia
QH301 Biology / biológia
Tartalmi leírás:
This article overviews the numerous immobilization methods available for various biocatalysts such as whole-cells, cell fragments, lysates or enzymes which do not require preliminary enzyme purification and introduces an advanced approach avoiding the costly and time consuming downstream processes required by immobilization of purified enzyme-based biocatalysts (such as enzyme purification by chromatographic methods and dialysis). Our approach is based on silica shell coated magnetic nanoparticles as solid carriers decorated with mixed functions having either coordinative binding ability (a metal ion complexed by a chelator anchored to the surface) or covalent bond-forming ability (an epoxide attached to the surface via a proper linker) enabling a single operation enrichment and immobilization of a recombinant phenylalanine ammonia-lyase from parsley fused to a polyhistidine affinity tag.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Sánta-Bell, Evelin and Molnár, Zsófia Klára and Varga, Andrea and Nagy, Flóra and Hornyánszky, Gábor and Paizs, Csaba and Balogh Weiser, Diána and Poppe, László (2019) “Fishing and hunting”– Selective immobilization of a recombinant phenylalanine ammonia-lyase from fermentation media. MOLECULES, 24 (22). ISSN 1420-3049
Kapcsolat:
MTMT:30920179 10.3390/molecules24224146