Bioactivity guided isolation of phytoestrogenic compounds from Cyclopia genistoides by the pER8: GUS reporter system
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Tartalom: http://real.mtak.hu/100726/
Archívum: REAL
Gyűjtemény: Status = Published
Subject = R Medicine / orvostudomány: RV Botanic, Thomsonian, and eclectic medicine / természetgyógyászat
Subject = R Medicine / orvostudomány: RM Therapeutics. Pharmacology / terápia, gyógyszertan
Type = Article
Cím:
Bioactivity guided isolation of phytoestrogenic compounds from Cyclopia genistoides by the pER8: GUS reporter system
Létrehozó:
Roza, Orsolya
Lai, W.-C.
Zupkó, István
Hohmann, Judit
Jedlinszki, Nikoletta
Csupor, Dezső
Kiadó:
Elsevier
Dátum:
2017
Téma:
RM Therapeutics. Pharmacology / terápia, gyógyszertan
RV Botanic, Thomsonian, and eclectic medicine / természetgyógyászat
Tartalmi leírás:
The popular South African herbal tea, honeybush, is made from several . Cyclopia species (family: . Fabaceae), amongst them . Cyclopia genistoides. Phytoestrogenic potential of . C. genistoides. has been recently reported, however bioactivity-guided isolation of compounds with estrogenic activity has not yet been performed.A transgenic plant system, . Arabidopsis thaliana pER8:GUS, was used to assay the estrogen-like activity of . C. genistoides. The quantitative determination of the active compounds in the fermented and non-fermented plant material was performed by HPLC. Subsequent bioactivity-guided fractionation led to the isolation of genistein, naringenin, isoliquiritigenin, luteolin, helichrysin B and 5,7,3',5'-tetrahydroxyflavanone, four of them first reported in the genus.Helichrysin B, naringenin and 5,7,3',5'-tetrahydroxyflavanone differed in quantity in the fermented and unfermented herbs, the fermented plant material contained two compounds with substantial estrogenic-like activity in higher concentration (naringenin and 5,7,3',5'-tetrahydroxyflavanone), whereas the less active helichrysin B was more abundant in the unfermented herb. The fractions as well as compounds inhibited the growth of human cancer cell lines A2780 and T47D.These results underline the phytoestrogenic activity of . C. genistoides and support the rationale to the fermentation process. © 2016 South African Association of Botanists.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Roza, Orsolya and Lai, W.-C. and Zupkó, István and Hohmann, Judit and Jedlinszki, Nikoletta and Csupor, Dezső (2017) Bioactivity guided isolation of phytoestrogenic compounds from Cyclopia genistoides by the pER8: GUS reporter system. SOUTH AFRICAN JOURNAL OF BOTANY, 110. pp. 201-207. ISSN 0254-6299
Kapcsolat:
MTMT:3106545 10.1016/j.sajb.2016.06.001