A critical evaluation of methods used for S-genotyping: from trees to DNA level
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Tartalom: https://ojs.lib.unideb.hu/IJHS/article/view/631
Archívum: IJHS
Gyűjtemény: Articles
Cím:
A critical evaluation of methods used for S-genotyping: from trees to DNA level
Létrehozó:
Halász, J.
Hegedűs, A.
Kiadó:
University of Debrecen
Dátum:
2006-04-19
Téma:
F-box
NEpHGE
PCR
Rosaceae
self-incompatibility
S-genotype
S-ribonuclease
Tartalmi leírás:
Fruit setting behaviour of fruit trees remains to be in the focus of plant breeders and growers. Realizing that most species (cherry, apple, pear etc.) are self-incompatible and certain cultivars are cross-incompatible, mutual fertility properties and their reliable determination are of great interest. This review gives a comprehensive description of all known S-genotyping procedures, i.e. the classical fruit set analysis after open field test crosses; pollen tube growth monitoring with fluorescent microscopy; stylar ribonuclease electrophoresis (using different types of isoelectric focusing and 2-dimension polyacrilamide gel electrophoresis); as well as the most recent polymerase chain reaction based DNA-level analyses and DNA sequencing. The review presented not only gives a compilation of the bases of the methods described but also provides a critical evaluation and a comparative characterization of their applicability.
Nyelv:
angol
Típus:
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Formátum:
application/pdf
Azonosító:
10.31421/IJHS/12/2/631
Forrás:
International Journal of Horticultural Science; Vol 12 No 2 (2006); 19-29.
1585-0404
10.31421/IJHS/12/2
Kapcsolat:
Létrehozó:
Copyright (c) 2018 International Journal of Horticultural Science