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Attempting Regeneration from Cultured Cotyledons and Plant Regeneration from Cotyledonary Nodes in Common Bean (Phaseolus vulgaris L.)

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Tartalom: https://ojs.lib.unideb.hu/IJHS/article/view/24
Archívum: IJHS
Gyűjtemény: Articles
Cím:
Attempting Regeneration from Cultured Cotyledons and Plant Regeneration from Cotyledonary Nodes in Common Bean (Phaseolus vulgaris L.)
Létrehozó:
Eissa Ahmed, E.
Bisztray, Gy.
Velich, I.
Kiadó:
University of Debrecen
Dátum:
1999-05-24
Téma:
Phaseolus vulgaris bean
cotyledonary node
shoot induction
Tartalmi leírás:
Dry seeds from two cultivars of common bean (Phaseolus vulgaris L.) were germinated on sterile cotton and sterile deionized distilled water. Cotyledonary node tissue of seedlings were cultured on Murashige and Skoog(MS)-based media supplemented with different combination of N6-benzyl-aminopurine (BAP) and indole-3-acetic acid (IAA), and benzyladenine (BA) and a-naphthaleneacetic acid (NAA). The results revealed that the regeneration percent and the average number of buds and shoots per explant were influenced by the type of explants and exogeneously added hormones. Multiple shoot induction on dry bean cotyledonary node that contain 4-5 mm from cotyledons and hypocotyl on a medium containing full concentration of MS inorganic salts supplemented with 0.5mg/1 BA and 0.1mg/1 NAA was feasible and the method can be applied in transformation experiments.  
Nyelv:
angol
Típus:
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Formátum:
application/pdf
Azonosító:
10.31421/IJHS/5/1-2/24
Forrás:
International Journal of Horticultural Science; Vol 5 No 1-2 (1999); 57-60.
1585-0404
10.31421/IJHS/5/1-2
Kapcsolat:
Létrehozó:
Copyright (c) 2018 International Journal of Horticultural Science