A myosin II nanomachine mimicking the striated muscle
  • Metaadatok
Tartalom: http://real.mtak.hu/84034/
Archívum: REAL
Gyűjtemény: Status = Published
Subject = Q Science / természettudomány: QH Natural history / természetrajz: QH301 Biology / biológia: QH3020 Biophysics / biofizika
Type = Article
Cím:
A myosin II nanomachine mimicking the striated muscle
Létrehozó:
Pertici, Irene
Bongini, Lorenzo
Melli, Luca
Bianchi, Giulio
Salvi, Luca
Bozó, Tamás
Kellermayer, Miklós
Kiadó:
Nature Publishing Group
Dátum:
2018
Téma:
QH3020 Biophysics / biofizika
Tartalmi leírás:
The contraction of striated muscle (skeletal and cardiac muscle) is generated by ATP-dependent interactions between the molecular motor myosin II and the actin filament. The myosin motors are mechanically coupled along the thick filament in a geometry not achievable by single-molecule experiments. Here we show that a synthetic one-dimensional nanomachine, comprising fewer than ten myosin II dimers purified from rabbit psoas, performs isometric and isotonic contractions at 2 mM ATP, delivering a maximum power of 5 aW. The results are explained with a kinetic model fitted to the performance of mammalian skeletal muscle, showing that the condition for the motor coordination that maximises the efficiency in striated muscle is a minimum of 32 myosin heads sharing a common mechanical ground. The nanomachine offers a powerful tool for investigating muscle contractile-protein physiology, pathology and pharmacology without the potentially disturbing effects of the cytoskeletal-and regulatory-protein environment.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Pertici, Irene and Bongini, Lorenzo and Melli, Luca and Bianchi, Giulio and Salvi, Luca and Bozó, Tamás and Kellermayer, Miklós (2018) A myosin II nanomachine mimicking the striated muscle. NATURE COMMUNICATIONS, 9 (1). p. 3532. ISSN 2041-1723
Kapcsolat:
MTMT:3411348; doi:10.1038/s41467-018-06073-9