A 60 kD heat-shock protein-like molecule interacts with the HIV transmembrane glycoprotein gp41

Speth, Cornelia

Prohászka, Zoltán

Mair, Mechthild

Stöckl, Gabriele

Zhu, Xiaojie

Jöbstl, Barbara

Füst, George

Dierich, Manfred P.

Elsevier

1999

QH3011 Biochemistry / biokémia

The heat-shock protein hsp60 is typically found in mitochondria. but, in smaller amounts, also in the cell cytoplasm and associated with the cell membrane. Since heat-shock proteins are known to interact with a variety of molecules and since purified HIV-1 particles were described to contain hsp60 molecules, we tested the possibility that a previously described putative receptor for HIV transmembrane protein gp41 is identical to hsp60. The gp41-binding human protein P62 was purified from H9 and Raji cell lysates by a gp41-coupled affinity column. We could show crossreactivity of both polyclonal and monoclonal anti-hsp60 antibodies with the purified P62. In addition we analyzed binding of P18, a soluble gp41 fragment harboring the extracellular domain (Env aa539-684), to recombinant hsp60. Hsp60 bound well to P18-coated ELISA plates whereas HIV-1 surface protein gp120 induced no binding of hsp60. Preincubation of hsp60 with gp41 abolished the binding. The possible role of this molecule as a cofactor in the pathogenesis of HIV disease is discussed.

angol

Article

PeerReviewed

info:eu-repo/semantics/article

text

http://real.mtak.hu/83098/1/1_s2.0_S0161589099000826_main_u.pdf

Speth, Cornelia and Prohászka, Zoltán and Mair, Mechthild and Stöckl, Gabriele and Zhu, Xiaojie and Jöbstl, Barbara and Füst, George and Dierich, Manfred P. (1999) A 60 kD heat-shock protein-like molecule interacts with the HIV transmembrane glycoprotein gp41. MOLECULAR IMMUNOLOGY, 36 (9). pp. 619-628. ISSN 0161-5890

http://real.mtak.hu/83098/

https://doi.org/10.1016/S0161-5890(99)00082-6

MTMT:1070642; doi:10.1016/S0161-5890(99)00082-6