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A 60 kD heat-shock protein-like molecule interacts with the HIV transmembrane glycoprotein gp41

  • Metaadatok
Tartalom: http://real.mtak.hu/83098/
Archívum: REAL
Gyűjtemény: Status = Published
Subject = Q Science / természettudomány: QH Natural history / természetrajz: QH301 Biology / biológia: QH3011 Biochemistry / biokémia
Type = Article
Cím:
A 60 kD heat-shock protein-like molecule interacts with the HIV transmembrane glycoprotein gp41
Létrehozó:
Speth, Cornelia
Prohászka, Zoltán
Mair, Mechthild
Stöckl, Gabriele
Zhu, Xiaojie
Jöbstl, Barbara
Füst, George
Dierich, Manfred P.
Kiadó:
Elsevier
Dátum:
1999
Téma:
QH3011 Biochemistry / biokémia
Tartalmi leírás:
The heat-shock protein hsp60 is typically found in mitochondria. but, in smaller amounts, also in the cell cytoplasm and associated with the cell membrane. Since heat-shock proteins are known to interact with a variety of molecules and since purified HIV-1 particles were described to contain hsp60 molecules, we tested the possibility that a previously described putative receptor for HIV transmembrane protein gp41 is identical to hsp60. The gp41-binding human protein P62 was purified from H9 and Raji cell lysates by a gp41-coupled affinity column. We could show crossreactivity of both polyclonal and monoclonal anti-hsp60 antibodies with the purified P62. In addition we analyzed binding of P18, a soluble gp41 fragment harboring the extracellular domain (Env aa539-684), to recombinant hsp60. Hsp60 bound well to P18-coated ELISA plates whereas HIV-1 surface protein gp120 induced no binding of hsp60. Preincubation of hsp60 with gp41 abolished the binding. The possible role of this molecule as a cofactor in the pathogenesis of HIV disease is discussed.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Speth, Cornelia and Prohászka, Zoltán and Mair, Mechthild and Stöckl, Gabriele and Zhu, Xiaojie and Jöbstl, Barbara and Füst, George and Dierich, Manfred P. (1999) A 60 kD heat-shock protein-like molecule interacts with the HIV transmembrane glycoprotein gp41. MOLECULAR IMMUNOLOGY, 36 (9). pp. 619-628. ISSN 0161-5890
Kapcsolat:
MTMT:1070642; doi:10.1016/S0161-5890(99)00082-6