A Cucumber Mosaic Virus Based Expression System for the Production of Porcine Circovirus Specific Vaccines
  • Metaadatok
Tartalom: http://real.mtak.hu/26589/
Archívum: REAL
Gyűjtemény: Status = Published
Subject = Q Science / természettudomány: QR Microbiology / mikrobiológia: QR355 Virology / víruskutatás
Subject = Q Science / természettudomány: QP Physiology / élettan
Subject = S Agriculture / mezőgazdaság: SB Plant culture / növénytermesztés
Subject = S Agriculture / mezőgazdaság: SV Veterinary science / állatorvostudomány
Type = Article
Cím:
A Cucumber Mosaic Virus Based Expression System for the Production of Porcine Circovirus Specific Vaccines
Létrehozó:
Gellért, Ákos
Salánki, Katalin
Tombácz, Kata
Tuboly, Tamás
Balázs, Ervin
Kiadó:
Public Library of Science
Dátum:
2012
Téma:
QP Physiology / élettan
QR355 Virology / víruskutatás
SB Plant culture / növénytermesztés
SV Veterinary science / állatorvostudomány
Tartalmi leírás:
Potential porcine circovirus type 2 (PCV2) capsid protein epitopes, suitable for expression on the surface of cucumber mosaic virus (CMV) particles were determined by a thorough analysis of the predicted PCV capsid protein structure. The ab initio protein structure prediction was carried out with fold recognition and threading methods. The putative PCV epitopes were selected on the basis of PCV virion models and integrated into the plant virus coat protein, after amino acid position 131. The recombinants were tested for infectivity and stability on different Nicotiana species and stable recombinant virus particles were purified. The particles were tested for their ability to bind to PCV induced porcine antibodies and used for specific antibody induction in mice and pigs. The results showed that PCV epitopes expressed on the CMV surface were recognized by the porcine antibodies and they were also able to induce PCV specific antibody response. Challenge experiment with PCV2 carried out in immunized pigs showed partial protection against the infection. Based on these results it was concluded that specific antiviral vaccine production for the given pathogen was feasible, offering an inexpensive way for the mass production of such vaccines.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Gellért, Ákos and Salánki, Katalin and Tombácz, Kata and Tuboly, Tamás and Balázs, Ervin (2012) A Cucumber Mosaic Virus Based Expression System for the Production of Porcine Circovirus Specific Vaccines. PLOS ONE, 7 (12). e52688. ISSN 1932-6203
Kapcsolat:
doi:10.1371/journal.pone.0052688