A genetically encoded sensor for visualizing leukotriene B4 gradients in vivo
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Tartalom: https://real.mtak.hu/246454/
Archívum: REAL
Gyűjtemény: Status = Published
Subject = Q Science / természettudomány: QR Microbiology / mikrobiológia
Subject = Q Science / természettudomány: QP Physiology / élettan
Type = Article
Cím:
A genetically encoded sensor for visualizing leukotriene B4 gradients in vivo
Létrehozó:
Tamás, Szimonetta Xénia
Roux, Benoit Thomas
Vámosi, Boldizsár
Dehne, Fabian Gregor
Török, Anna
Fazekas, László
Enyedi, Balázs
Kiadó:
Nature Publishing Group
Dátum:
2023
Téma:
QP Physiology / élettan
QR Microbiology / mikrobiológia
Tartalmi leírás:
Leukotriene B 4 (LTB 4 ) is a potent lipid chemoattractant driving inflammatory responses during host defense, allergy, autoimmune and metabolic diseases. Gradients of LTB 4 orchestrate leukocyte recruitment and swarming to sites of tissue damage and infection. How LTB 4 gradients form and spread in live tissues to regulate these processes remains largely elusive due to the lack of suitable tools for monitoring LTB 4 levels in vivo. Here, we develop GEM-LTB 4 , a genetically encoded green fluorescent LTB 4 biosensor based on the human G-protein-coupled receptor BLT1. GEM-LTB 4 shows high sensitivity, specificity and a robust fluorescence increase in response to LTB 4 without affecting downstream signaling pathways. We use GEM-LTB 4 to measure ex vivo LTB 4 production of murine neutrophils. Transgenic expression of GEM-LTB 4 in zebrafish allows the real-time visualization of both exogenously applied and endogenously produced LTB 4 gradients. GEM-LTB 4 thus serves as a broadly applicable tool for analyzing LTB 4 dynamics in various experimental systems and model organisms.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Tamás, Szimonetta Xénia and Roux, Benoit Thomas and Vámosi, Boldizsár and Dehne, Fabian Gregor and Török, Anna and Fazekas, László and Enyedi, Balázs (2023) A genetically encoded sensor for visualizing leukotriene B4 gradients in vivo. NATURE COMMUNICATIONS, 14. No. 4610. ISSN 2041-1723
Kapcsolat:
MTMT:34085672 10.1038/s41467-023-40326-6
Létrehozó:
cc_by