3D cell segregation geometry and dynamics are governed by tissue surface tension regulation

Méhes, Elod

Mones, Enys

Varga, Máté

Zsigmond, Áron

Biri-Kovács, Beáta

Nyitray, László

Barone, Vanessa

Krens, Gabriel

Heisenberg, Carl-Philipp

Vicsek, Tamás

2023

Tissue morphogenesis and patterning during development involve the segregation of cell types. Segregation is driven by differential tissue surface tensions generated by cell types through controlling cell-cell contact formation by regulating adhesion and actomyosin contractility-based cellular cortical tensions. We use vertebrate tissue cell types and zebrafish germ layer progenitors as in vitro models of 3-dimensional heterotypic segregation and developed a quantitative analysis of their dynamics based on 3D time-lapse microscopy. We show that general inhibition of actomyosin contractility by the Rho kinase inhibitor Y27632 delays segregation. Cell type-specific inhibition of non-muscle myosin2 activity by overexpression of myosin assembly inhibitor S100A4 reduces tissue surface tension, manifested in decreased compaction during aggregation and inverted geometry observed during segregation. The same is observed when we express a constitutively active Rho kinase isoform to ubiquitously keep actomyosin contractility high at cell-cell and cell-medium interfaces and thus overriding the interface-specific regulation of cortical tensions. Tissue surface tension regulation can become an effective tool in tissue engineering.

info:eu-repo/semantics/article

application/pdf

http://hdl.handle.net/10831/107980

doi:https://doi.org/10.1038/s42003-023-05181-7

elte:001042544100001

elte:85166597618

elte:34087969

elte:1

elte:COMMUN BIOL

elte:COMMUNICATIONS BIOLOGY

elte:6

elte:37542157

elte:10094780

LOMS: https://edit.elte.hu/xmlui/bitstream/10831/107980/1/34087969.pdf

info:eu-repo/semantics/openAccess