| Tartalom: |
http://hdl.handle.net/10832/2920 |
| Archívum: |
HUVETA |
| Gyűjtemény: |
Magyar Állatorvosok Lapja
2015
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| Cím: |
A Dirofilaria immitis és Dirofilaria repens mikrofiláriák fénymikroszkópos vizsgálata - 1. rész: A mikrofiláriák felismerése a különféle mintákban
Investigation of microfilariae of Dirofilaria immitis and Dirofilaria repens by light microscope - Part 1: Recognition of microfilariae in different samples
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| Létrehozó: |
Majoros, Gábor
Juhász, Alexandra
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| Kiadó: |
Magyar Állatorvosok Lapja
|
| Dátum: |
2021-04-30T13:00:26Z
2021-04-30T13:00:26Z
2015-03
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| Tartalmi leírás: |
SUMMARY
The authors suggest easy methods for veterinarians with small animal practice to
detect the presence of microfilariae produced by Dirofilaria immitis and Dirofilaria repens
nematodes and to recognize their embryonic larvae (prelarvae). The presented
simple light microscopy techniques help to reveal as many subclinical infections
as possible, and not only in living animal, but also in carcasses of dead ones.
As the actively moving creatures in blood can be detected easier than motionless
ones, the finding of the microfilariae is promoted by using such way of haemolysis
that does not kill them. Instead of the traditional Knott’s method therefore, they
suggest to use sodium lauryl sulphate for haemolysis and Nile blue for staining
living and dead microfilariae both in order to see them better. Clotted blood of the
dead animals can be investigated easily for microfilariae if a bit of clot is immersed
into a dark solution of alkaline histological dye (i.e. methylene or Nile blue)
then it is pressed apart on microscopic slide.
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| Nyelv: |
magyar
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| Típus: |
Article
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| Azonosító: |
Magyar Állatorvosok Lapja 137(3), 173-180. (2015)
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