| Tartalom: |
http://unipub.lib.uni-corvinus.hu/2194/ |
| Archívum: |
Corvinus Kutatások |
| Gyűjtemény: |
Status = Published
Subject = Viticulture
Type = Article
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| Cím: |
Silencing Agrobacterium oncogenes in transgenic grapevine results in strain-specific crown gall resistance
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| Létrehozó: |
Galambos, Anikó
Zok, Anikó
Kuczmog, Anett
Oláh, Róbert
Putnoky, Péter
Szegedi, Ernő
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| Kiadó: |
Springer
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| Dátum: |
2013
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| Téma: |
Viticulture
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| Tartalmi leírás: |
Crown gall disease of grapevine induced by Agrobacterium vitis or Agrobacterium tumefaciens causes serious
economic losses in viticulture. To establish crown gall-resistant lines, somatic proembryos of Vitis
berlandieri × V. rupestris cv. 'Richter 110' rootstock were transformed with an oncogene-silencing transgene
based on iaaM and ipt oncogene sequences from octopine-type, tumor-inducing (Ti) plasmid pTiA6. Twentyone
transgenic lines were selected, and their transgenic nature was confirmed by polymerase chain reaction
(PCR). These lines were inoculated with two A. tumefaciens and three A. vitis strains. Eight lines showed
resistance to octopine-type A. tumefaciens A348. Resistance correlated with the expression of the silencing
genes. However, oncogene silencing was mostly sequence specific because these lines did not abolish
tumorigenesis by A. vitis strains or nopaline-type A. tumefaciens C58.
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| Típus: |
Article
PeerReviewed
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| Formátum: |
application/pdf
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| Azonosító: |
Galambos, Anikó and Zok, Anikó and Kuczmog, Anett and Oláh, Róbert and Putnoky, Péter and Szegedi, Ernő (2013) Silencing Agrobacterium oncogenes in transgenic grapevine results in strain-specific crown gall resistance. Plant Cell Reports, 32 (11). pp. 1751-1757. ISSN 0721-7714
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| Kapcsolat: |
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